C06 - Empowering life science by microfluidic hyperpolarized NMR

Nuclear magnetic resonance offers atomic-level resolution of molecular structure and dynamcis, and can determine residue-resolved ligand-protein affinities via titration experiments. On-chip microfluidics support such titration experiments with minimal sample volumes, but the low concentration sensitivity of microNMR limits their applicability to concentrations and affinities in the high mM range. Within this project we will combine automated dilution-free bullet-DNP with on-chip titration to determine affinities using protein and ligand concentrations in the nanomolar to low micromolar range.

Team